Publications by authors named "S SowjanyaKumari"
Article Synopsis
- Timely diagnosis of leptospirosis is crucial for preventing severe health outcomes in both humans and animals, and current diagnostic methods have limitations that necessitate new approaches.
- This study explores a novel diagnostic tool using a recombinant lipoprotein (rErpY-LIC11966) in a latex agglutination test (LAT), which was developed and tested for its effectiveness in diagnosing animal leptospirosis.
- The rErpY-LAT demonstrated high diagnostic performance, with 90.6% sensitivity, 89.1% specificity, and 90% overall accuracy, making it a promising field-applicable option for early serodiagnosis of the disease.
Article Synopsis
- Leptospirosis and dengue are major health concerns in the Dakshina Kannada district of Karnataka, India, where both diseases are understudied and have significant health risks.
- The study aimed to determine the prevalence of these diseases among people with febrile illnesses and assess co-infection rates during 2020-2021.
- Results indicated that 21% of febrile cases had leptospirosis and 10% had dengue, with a low co-infection rate of 1.3%, emphasizing the need for better public health strategies and diagnostic tools in the region.
Leptospirosis is a significant zoonotic disease affecting livestock, leading to reproductive issues and economic losses. Despite its endemic status in India, research has predominantly focused on coastal regions, leaving the North Eastern Region (NER) underexplored. This study aims to investigate the seroprevalence and serogroup distribution of leptospirosis in livestock across Assam, a major state in the North Eastern Region (NER) of India.
View Article and Find Full Text PDFThe study describes the expression of recombinant truncated nucleocapsid protein (NP) of peste des petits ruminants (PPR) virus in the baculovirus system (PPRV-rBNP) and its potential application as a diagnostic antigen in ELISA for diagnosis of PPR in sheep and goats. The PPRV N-terminal immunogenic region (1-266 aa) of the NP coding sequence was amplified and cloned into the pFastBac HT A vector. The PPRV-rBNP with a molecular weight of ∼30 kDa was expressed in an insect cell system using generated recombinant baculovirus through Bac-to-Bac® Baculovirus Expression System.
View Article and Find Full Text PDFArticle Synopsis
- - The study assessed the effectiveness of a new Avidin-Biotin recombinant nucleoprotein competitive ELISA (ABrC-ELISA) for detecting antibodies against peste des petits ruminants (PPR) in sheep and goats, highlighting its capability for serosurveillance and monitoring.
- - The ABrC-ELISA utilized a recombinant PPR virus nucleoprotein produced in E. coli and showed promising diagnostic performance, with a sensitivity of 87.2% and specificity of 92.0% when tested on over 1,400 animal sera samples.
- - Results indicate that ABrC-ELISA is a reliable diagnostic tool for PPRV detection, potentially usable in field conditions in regions working towards