MIBiG 4.0: advancing biosynthetic gene cluster curation through global collaboration.

Specialized or secondary metabolites are small molecules of biological origin, often showing potent biological activities with applications in agriculture, engineering and medicine. Usually, the biosynthesis of these natural products is governed by sets of co-regulated and physically clustered genes known as biosynthetic gene clusters (BGCs). To share information about BGCs in a standardized and machine-readable way, the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard and repository was initiated in 2015.

Identifying Learning Preferences and Strategies in Health Data Science Courses: Systematic Review.

Background: Learning and teaching interdisciplinary health data science (HDS) is highly challenging, and despite the growing interest in HDS education, little is known about the learning experiences and preferences of HDS students.

Objective: We conducted a systematic review to identify learning preferences and strategies in the HDS discipline.

Methods: We searched 10 bibliographic databases (PubMed, ACM Digital Library, Web of Science, Cochrane Library, Wiley Online Library, ScienceDirect, SpringerLink, EBSCOhost, ERIC, and IEEE Xplore) from the date of inception until June 2023.

The Influence of nc-AlCrTiN/α-BN Coatings on Increasing the Durability of WC/Co Cutting Inserts in the Inconel Alloy Machining Process.

Anti-wear coatings obtained through PVD methods may significantly increase the durability of cutting tools by impacting their wear mechanisms. This study presents and discusses the results of studies on the impact of the thermal conductivity of PVD coatings on the intensity of the built-up edge (BUE) and built-up layer (BUL) formation in Inconel 600 alloy machining processes. The authors determine the microstructure, phase structure, mechanical properties (hardness, Young's modulus, and adhesion), and thermal conductivity of different PVD coatings selected for the purpose of the study and varying in terms of conductivity-i.

Biosynthesis of the bacterial antibiotic 3,7-dihydroxytropolone through enzymatic salvaging of catabolic shunt products.

The non-benzenoid aromatic tropone ring is a structural motif of numerous microbial and plant natural products with potent bioactivities. In bacteria, tropone biosynthesis involves early steps of the widespread CoA-dependent phenylacetic acid (paa) catabolon, from which a shunt product is sequestered and surprisingly further utilized as a universal precursor for structurally and functionally diverse tropone derivatives such as tropodithietic acid or (hydroxy)tropolones. Here, we elucidate the biosynthesis of the antibiotic 3,7-dihydroxytropolone in Actinobacteria by pathway reconstitution using paa catabolic enzymes as well as dedicated downstream tailoring enzymes, including a thioesterase (TrlF) and two flavoprotein monooxygenases (TrlCD and TrlE).