Visualization as irritation: producing knowledge about medieval courts through uncertainty.

Visualizations are ubiquitous in data-driven research, serving as both tools for knowledge production and genuine means of knowledge communication. Despite criticisms targeting the alleged objectivity of visualizations in the digital humanities (DH) and reflections on how they may serve as representations of both scholarly perspective and uncertainty within the data analysis pipeline, there remains a notable scarcity of in-depth theoretical grounding for these assumptions in DH discussions. It is our understanding that only through theoretical foundations such as basic semiotic principles and perspectives on media modality one can fully assess the use and potential of visualizations for innovation in scholarly interpretation.

The influence of temperature treatment before cryopreservation on the viability and potency of cryopreserved and thawed CD34 and CD45 cord blood cells.

Background: Hematopoietic stem cell (HSC) viability and potency is crucial for qualified cord blood (CB) transplants. This study analyzes time and temperature condition before cryopreservation for the viability of CD34/CD45 cells after cryopreservation.

Methods: Cell viabilities were determined by antibody co-staining with 7-aminoactinomycin D detecting necrotic cells, and subsequent flow cytometric analysis.

Cord blood collection and processing with hydroxyethyl starch or non-hydroxyethyl starch.

Background: Collection and processing characteristics influencing quality of cord blood (CB) units play an essential role to cord blood banks (CBBs). At many CBBs, volume reduction is performed using hydroxyethyl starch (HES) and the Sepax (Biosafe) automated cell processing system. Due to the withdrawal of HES from the European market, a validation of the nonHES protocol was performed.

Differential roles for octanoylated and decanoylated ghrelins in regulating appetite and metabolism.

Since its identification in 1999, ghrelin has been identified in all vertebrate groups. The "active core" of ghrelin is highly conserved among vertebrates, suggesting its biological activity to be also conserved. In fish, both acylated forms of ghrelin have been identified; however, the ratio of the ghrelin-C8 to ghrelin-C10 is not as great as observed in mammals.

Glucose regulates ghrelin, neuropeptide Y, and the GH/IGF-I axis in the tilapia, Oreochromis mossambicus.

In general, a fish's ability to clear glucose is sluggish in relation to mammals, which has lead to the idea that fish are glucose intolerant. It has been reported that circulating glucose levels do fluctuate in response to environmental challenges. Recent reports suggest that glucose may function as a metabolic signal regulating 'glucosensors' in the brain in fish, as has been reported in mammals.