[Effect of the admixture of tetracycline and nadifloxacin ointments on their stability and their antibacterial activity].

Impetigo contagiosa staphylogenes is commonly treated by administering a combination of nadifloxacin and tetracycline ointments. However, it is not clear whether nadifloxacin and tetracycline are stable after mixing. The purpose of this study was to evaluate the stability of these agents in combination.

Identification and characterization of beta-D-galactosyl-transferase in chick corneas.

Purpose: To purify beta-galactosyltransferase, which is involved in the biosynthesis of keratan sulfate, from 2-day-old chick corneas.

Methods: The activity was assayed using pyridylaminated GlcNAcbeta1-3Galbeta1-4Glc as acceptor substrate.

Results: The beta-galactosyltransferase did not bind to several ion exchange and affinity columns.

Comparative analysis of proteoglycans synthesized by chick corneal stromal cells in cell culture and organ culture.

Proteoglycans (PGs) synthesized by chick corneal stromal cells in cell culture and organ culture were metabolically radiolabelled with [35S]sulfate (for glycosaminoglycans) and [3H]leucine (for core proteins). Media, cell extracts and organ extracts from cultures were chromatographed on DEAE-Sephacel columns and separated into three fractions: the pass-through fraction (Fraction 1: nonsulfated PGs, hardly sulfated PGs, or glycoproteins with some oligosaccharides), the fraction eluted with a low salt concentration (Fraction 2: undersulfated PGs), and the fraction eluted with a high salt concentration (Fraction 3: highly sulfated PGs). The PG compositions of each fraction of cell culture and organ culture were then compared.