Four new triterpenoid saponins from the roots of Bupleurum rigidum.

Five triterpenoid saponins [buddlejasaponin IV, sandrosaponins VII (1), VIII (2), IX (3), and X (4)] were isolated from an n-BuOH extract of the roots of Bupleurum rigidum. Sandrosaponins VII-X (1-4) are new compounds, and their structures were established by 1D and 2D NMR techniques, FABMS, and chemical methods.

Minor sulfated saikosaponins from the aerial parts of Bupleurum rigidum L.

Five new sulfated saikosaponins (Sandrosaponins II-VI) were isolated as minor components from the aerial parts of Bupleurum rigidum L. Their structures have been established by 1D and 2D-NMR techniques, FABMS, and chemical methods. ,

A sulfated saponin from bupleurum rigidum.

A new sulfated triterpene glycoside with the sulfate group located in an unusual position in the carbohydrate moiety, was isolated from the MeOH extract of the aerial parts of Bupleurum rigidum. This compound was identified by a combination of chemical degradation and spectral methods as 3beta,16beta,23-trihydroxy-13, 28-epoxyolean-11-en-3beta-yl-beta-D-glucopyranosyl-(1-->2)[4-sulfate- beta-D-glucopyranosyl-(1-->3)]-beta-D-fucopyranoside (sandrosaponin I) (1). In addition, the known compound 3beta,16beta, 23-trihydroxy-13, 28-epoxyolean-11-en-3beta-yl-beta-D-glucopyranosyl-(1-->2)[beta-D-glu copyranosyl-(1-->3)]beta-D-fucopyranoside (2) was isolated in the present investigation.

In vivo and in vitro antiinflammatory activity of saikosaponins.

Buddlejasaponin I and saikosaponin 1 and 2, biologically active compounds from Scrophularia scorodonia and Bupleurum rigidum respectively, exert potent in vivo antiinflammatory effects on mouse ear edema induced by phorbol myristate acetate (PMA). The effects of these compounds on swelling and other inflammatory parameters are described. In screening for in vitro effects of saikosaponins on cellular systems generating cyclooxygenase (COX) and lipoxygenase (LOX) metabolites, we observed that most saikosaponins showed a significant effect.