Publications by authors named "S Saisingtong"
Article Synopsis
- The study focused on improving the process of doubling chromosomes in wheat through anther culture using colchicine.
- Optimal concentrations of colchicine were found to be 100 and 1000 mg/l, with significant improvements in doubling indices and reduced embryo structures.
- The best results for chromosome doubling occurred when colchicine was applied 20 days after inoculation, with a notable increase in success when used for 3 days.
Efficient methods of chromosome doubling are critical for the production of microspore-derived, doubled-haploid (=DH) plants, especially if, as in maize anther culture, spontaneous chromosome doubling occurs infrequently. In the present study, colchicine (5-1000 mg/l) was added to the induction medium and maize anthers were incubated in the colchicine-containing medium for different durations (1-7 days). In order to improve overall anther culture response, the culture temperature was adjusted to 14°C during the first 7 days.
View Article and Find Full Text PDF