Femtosecond laser modified metal surfaces alter biofilm architecture and reduce bacterial biofilm formation.

Biofilm formation, or microfouling, is a basic strategy of bacteria to colonise a surface and may happen on surfaces of any nature whenever bacteria are present. Biofilms are hard to eradicate due to the matrix in which the bacteria reside, consisting of strong, adhesive and adaptive self-produced polymers such as eDNA and functional amyloids. Targeting a biofilm matrix may be a promising strategy to prevent biofilm formation.

eDNA Inactivation and Biofilm Inhibition by the PolymericBiocide Polyhexamethylene Guanidine Hydrochloride (PHMG-Cl).

The choice of effective biocides used for routine hospital practice should consider the role of disinfectants in the maintenance and development of local resistome and how they might affect antibiotic resistance gene transfer within the hospital microbial population. Currently, there is little understanding of how different biocides contribute to eDNA release that may contribute to gene transfer and subsequent environmental retention. Here, we investigated how different biocides affect the release of eDNA from mature biofilms of two opportunistic model strains ATCC 27853 (PA) and ATCC 25923 (SA) and contribute to the hospital resistome in the form of surface and water contaminants and dust particles.

[Neutrophil functional activity in rats with parodontal inflammatory process at the background of reduced thyroid gland function].

Influence of reduced thyroid gland function upon peripheral blood neutrophil functional activity in Wistar rats with acute mechanical trauma of gingival was studied. Thyroid insufficiency was simulated by daily intraoral Mercazole administration in the doze of 25 mg/kg during 21 days. The parodontal inflammatory process in rats with reduced thyroid gland function was characterized by reduced indicators of spontaneous and induced HCT-tests, by lower activity of posttraumatic inflammatory purulent processes in comparison with animals with euthyroid status.

[Genes of insecticidal crystal proteins with dual specificity in Bacillus thuringiensis strains, isolated in the Crimea territory].

The insecticidal crystal proteins of 15 B. thuringiensis strains, isolated in the Crimea territory that are toxical for some Lepidoptera and Colorado potato beetle larvae were identified by PAGE electrophoresis. Ten strains produced the crystal proteins with high molecular weight (> 120 kD).

[The construction and use of inosine-containing primers for searching for and identifying the genes of insecticidal crystal proteins in Bacillus thuringiensis].

The specific to 3 types of Cry genes primers containing inosine were constructed to find crystal insecticidal protein Bacillus thuringiensis genes using PCR. A number of new B. thuringiensis strains isolated in Ukraine were investigated using these PCR primers.