Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose.

2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG.

Cardiac prehabilitation, rehabilitation and education in first-time atrial fibrillation (AF) ablation (CREED AF): Study protocol for a randomised controlled trial.

Background: Atrial fibrillation (AF) is associated with significant morbidity/mortality. AF-ablation is an increasingly used treatment. Currently, first-time AF-ablation success is 40-80% at 1-year, depending on individual factors.

-BuNI/KSO-MEDIATED C-N COUPLING BETWEEN ALDEHYDES AND AMIDES.

-BuNI/KSO mediated C-N coupling between aldehydes and amides is reported. A strong electronic effect is observed on the aromatic aldehyde substrates. The transformylation from aldehyde to amide takes place exclusively when an aromatic aldehyde bears electron-donating groups at either the or position of the formyl group, while the cross-dehydrogenative coupling dominates in the absence of these groups.

-BuNI/KSO Mediated Csp-Csp Bond Cleavage - Transformylation from -Anisaldehyde to Primary Amides.

-BuNI/KSO mediated transformylation from -anisaldehyde to primary amides is reported. The mechanistic studies suggest the reaction occurs via a single electron transfer pathway. Based on the DFT electronic structure calculations of various reaction pathways, the most plausible mechanism involves the formation of a phenyl radical cation and an arenium ion as the key intermediates.