Publications by authors named "S S Phugare"
Multivalent meningococcal conjugate vaccines are a significant focus for the scientific community in light of the WHO's mission to defeat meningitidis by 2030. Well-known meningococcal vaccines such as MenAfriVac, Nimenrix, Menveo, and MenQuadfi are licensed in various parts of the world and have been successful. Recently, the World Health Organization (WHO) qualified MenFive (meningococcal A, C, Y, W, and X) conjugate vaccine, further enhancing the battery of vaccines against meningitis.
View Article and Find Full Text PDFMolecular size distribution in pentavalent (A, C, Y, W, X) meningococcal polysaccharide conjugate vaccine by HPSEC-UV-MALS-RI method- a conceivable stability indicating parameter.
J Chromatogr B Analyt Technol Biomed Life Sci
April 2024
Article Synopsis
- * A new method was developed to analyze the MSD of MenFive, which faced challenges due to the complexity of the vaccine's composition and various testing conditions, including different columns and temperatures.
- * Results showed a wide range of molecular sizes (200 to 19,000 kDa), with the most common size between 1,000 and 8,000 kDa, indicating that understanding MSD helps in assessing the effectiveness and consistency of the vaccine.
Article Synopsis
- - A new DOC-HCl method was developed for estimating free polysaccharide content in Meningococcal serogroups A, C, Y, W, and X within pentavalent vaccines, offering a practical alternative to the ultra-filtration technique.
- - Two specific DOC-HCl formulations were created for different serogroups with varying hydrochloric acid concentrations, ensuring effective protein-DOC complex precipitation and enhanced accuracy in polysaccharide measurement.
- - The method demonstrated robust performance with good recovery rates (70-130%), low variability (2-14%), and no interference from assay components, making it suitable for quality control in multivalent vaccines.
Meningococcal vaccines have been developed over the years, to control outbreaks of meningitis. There are 12 immunologically distinct serogroups of which, A, B, C, W, Y and X are predominant and invasive in nature. Meningococcal vaccines can be sorted out as, polysaccharide vaccines, polysaccharide protein conjugate vaccines or protein based (independent of polysaccharides) vaccines.
View Article and Find Full Text PDFArticle Synopsis
- An alkaline chitinase was isolated and purified from the bacterium Paenibacillus pasadenensis NCIM 5434 found in the alkaline soil of Lonar Lake using ammonium sulfate precipitation and DEAE cellulose column chromatography, achieving 8.87-fold purification with 24.96% yield.
- Molecular analysis showed the enzyme has a molecular weight of about 35 kDa, with kinetic parameters indicating a Km of 6.25 mg/ml and Vmax of 434.78 µM for colloidal chitin, operating optimally at pH 10 and 37°C.
- The enzyme displays notable antifungal activity against Penicillium and Aspergillus, suggesting its potential