X-ray crystallography is one of the leading tools to analyze the 3-D structure, and therefore, function of proteins and other biological macromolecules. Traditional methods of mounting individual crystals for X-ray diffraction analysis can be tedious and result in damage to fragile protein crystals. Furthermore, the advent of multi-crystal and serial crystallography methods explicitly require the mounting of larger numbers of crystals.
View Article and Find Full Text PDFThis Letter presents complex coacervation between the biopolymer diethylaminoethyl dextran hydrochloride (DEAE-Dex) and carbon dots. The formation of these coacervates was dependent on both DEAE-Dex concentration and solution ionic strength. Fluorescence spectroscopy revealed that the blue fluorescence of the carbon dots was unaffected by coacervation.
View Article and Find Full Text PDFBackground: Thiotepa-based autologous stem cell transplantation (ASCT) improves survival in primary central nervous system lymphoma (PCNSL), but > 30% of patients are unable to undergo ASCT following commonly used intensive induction regimens.
Methods: This retrospective population-based study included consecutive patients ≥ 18 years old with PCNSL who were intended for ASCT in Alberta, Canada between 2011 and 2022. A reduced-intensity induction protocol was further abbreviated in 2018 to decrease toxicity and expediate ASCT by incorporating rituximab, procarbazine, and only 2 doses of high-dose methotrexate and 1 cycle of high-dose cytarabine before consolidation with thiotepa-busulfan conditioning.
Cogn Res Princ Implic
December 2024