Investigation of Factors Affecting COVID-19 and Sixth Wave Management Using a System Dynamics Approach.

The COVID-19 pandemic has plunged the world into a health and economic crisis never seen before since the Spanish flu pandemic in 1918. The closure of schools and universities, the banning of rallies, and other social distancing in countries have been done to disrupt the transmission of the virus. Governments have planned to reduce restrictions on corona management by implementing vaccination programs.

[Conformation of adenosine deaminase in complexes with inhibitors: application of selective quenching of fluorescence emission].

The effect of inhibitors, 1-deazaadenosine (1-dAdo) and erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA), on the conformation of adenosine deaminase was studied using the method of selective quenching of fluorescence emission by acrylamide, I- and Cs+. Both in free adenosine deaminase and in its complexes with the inhibitors, the wavelength maxima and half-width of the emission characterize the environment of fluorescing tryptophan residues in adenosine deaminase as weak polar with limited access to solvent. The formation of complexes with the ground state inhibitors used did not quench or change the main emission characteristics of tryptophan fluorescence in adenosine deaminase.

Kinetic and structural analysis of the inhibition of adenosine deaminase by acetaminophen.

Kinetic and thermodynamic studies have been made on the effect of acetaminophen on the activity and structure of adenosine deaminase in 50 mM sodium phosphate buffer pH 7.5, at two temperatures of 27 and 37 degrees C using UV spectrophotometry, circular dichroism (CD) and fluorescence spectroscopy. Acetaminophen acts as a competitive inhibitor at 27 degrees C (Ki = 126 microM) and an uncompetitive inhibitor at 37 degrees C (Ki = 214 microM).

[Activity of pleural fluid adenosine deaminase in tuberculous pleurisy].

Examining the activity of adenosine deaminase in the pleural fluids of 69 patients with tuberculous pleurisy of various etiology from the clinics of Armenia indicated that it was greater than the threshold value of 20 U/L in 95.7 of 47 patients with tuberculous pleurisy. The specificity of this parameter for this disease was 0.

[Modification of the histidine in rat skeletal muscle deaminase by diethylpyrocarbonate].

Diethylpyrocarbonate inactivated rat skeletal muscle AMP deaminase in 10 mM phosphate buffer, pH 6.5, at 23 degrees with the second order rate constant of 580 M-1.min-1.