Magnetic solid-phase extraction method with modified magnetic ferroferric oxide nanoparticles in a deep eutectic solvent and high-performance liquid chromatography used for the analysis of pharmacologically active ingredients of Epimedium folium.

A novel magnetic adsorption nanomaterial was synthesized by the copolymerization of a deep eutectic solvent (DES) and chitosan (CS)-modified FeO particles. This material was proposed for the magnetic solid-phase extraction (MSPE) of flavonoids from an aqueous extract solution of herbal Epimedium folium without pretreatment. Coupled with high-performance liquid chromatography (HPLC), a rapid, environmentally friendly, and efficacious method was established and successfully applied for the enrichment, separation, and quantification of five quality marker flavonoids (viz.

The analytical performance of six urine drug screens on cobas 6000 and ARCHITECT i2000 compared to LC-MS/MS gold standard.

Background: Immunoassays provide a rapid tool for the screening of drugs-of-abuse (DOA). However, results are presumptive and confirmatory testing is warranted. To reduce associated cost and delay, laboratories should employ assays with high positive and negative predictive values (PPVs and NPVs).

Differential metabolomics networks analysis of menopausal status.

Menopause is an endocrine-related transition that induces a number of physiological and potentially pathological changes in middle-aged and elderly women. The intention of this research was to investigate the influence of menopause on the intricate relationships between major biochemical metabolites. The study involved metabolic profiling of 186 metabolic markers measured in blood plasma collected from 120 healthy female participants.

Baseline urinary KIM-1 concentration in detecting acute kidney injury should be interpreted with patient pre-existing nephropathy.

Objectives: To determine whether pre-existing nephropathy impacts urinary KIM-1 levels, urinary KIM-1 were measured in patients with normal kidney filtration function but either with or without proteinuria. The reference intervals of urinary KIM-1 in adults with normal kidney filtration function but without urine proteinuria were established.

Design And Methods: 188 urine samples were obtained from adults with normal kidney filtration.

Differential metabolomics analysis allows characterization of diversity of metabolite networks between males and females.

Females and males are known to have different abilities to cope with stress and disease. This study was designed to investigate the effect of sex on properties of a complex interlinked network constructed of central biochemical metabolites. The study involved the blood collection and analysis of a large set of blood metabolic markers from a total of 236 healthy participants, which included 140 females and 96 males.