Publications by authors named "S Rebuffat"

Article Synopsis
  • Microcins are antimicrobial peptides with diverse structures and actions that specifically target closely related bacterial strains, making them a potential alternative to traditional antibiotics by minimizing the risk of resistance spread and harm to beneficial bacteria.
  • A study examined resistance mechanisms to four types of microcins in a collection of 54 bacterial strains, finding that many resistant strains had mutations in genes critical for microcin uptake and function.
  • While a genome-wide association study did not show strong links, some resistance-associated genes were identified relating to stress responses, biofilm formation, transport, and immunity, along with metabolic pathway mutations in resistant strains.
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Covering: 1992 up to 2023Since their discovery, lasso peptides went from peculiarities to be recognized as a major family of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products that were shown to be spread throughout the bacterial kingdom. Microcin J25 was first described in 1992, making it one of the earliest known lasso peptides. No other lasso peptide has since then been studied to such an extent as microcin J25, yet, previous review articles merely skimmed over all the research done on this exceptional lasso peptide.

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Nonsense mutations are responsible for around 10% of cases of genetic diseases, including cystic fibrosis. 2,6-diaminopurine (DAP) has recently been shown to promote efficient readthrough of UGA premature stop codons. In this study, we show that DAP can correct a nonsense mutation in the Cftr gene in vivo in a new CF mouse model, in utero, and through breastfeeding, thanks, notably, to adequate pharmacokinetic properties.

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Article Synopsis
  • The COMET biobank project collects special tissue and blood samples from overweight patients to help with research on insulin sensitivity.
  • They set up a system to make sure all the samples are good quality and that the collection process is done the right way.
  • The results showed that the samples were collected properly, with good quality of DNA, RNA, and tissues, ensuring the project can be used for scientific studies safely.
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Lacticaseicin 30 is one of the five bacteriocins produced by the Gram-positive CNCM I-5369. This 111 amino acid bacteriocin is noteworthy for being active against Gram-negative bacilli including strains resistant to colistin. Prediction of the lacticaseicin 30 structure using the Alphafold2 pipeline revealed a largely helical structure including five helix segments, which was confirmed by circular dichroism.

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