[Effect of temperature on the synthesis of protein and individual amino acids by the thermotolerant yeast Candida tropicalis on media with hydrocarbons].

Synthesis of protein and amino acids by the thermotolerant yeast Candida tropicalis K-41 was studied at various temperatures of cultivation during growth on media with hydrocarbons in conditions of continuous cultivation. The content of protein in the cells decreased by 6% with an increase in growth temperature by 10 degrees C (from 29 to 39 degrees C) while excretion of free amino acids into the medium increased. The cultural broth of the cells grown at 39 degrees C contained up to 8 amino acids, with alanine, aspartic acid, serine and glycine prevailing.

[Effect of carbon source and cultivation temperature on growth limitation of Candida tropicalis by biotin].

The effect of biotin on the specific growth rate of Candida tropicalis was studied on media with various sources of carbon nutrition at temperatures of 29 and 39degreesC. The yeast was found to require 3--5 times less biotin on the medium with paraffin than on the medium with glucose and hexodecane, and 3--4 times more biotin on the same substrate with an increase in temperature. The growth rate increased by 36--40% on liquid paraffins containing 0.