Publications by authors named "S R S Prakki"
Background: An increased incidence of Pseudomonas aeruginosa in microbiological surveillance (MS) cultures from gastrointestinal endoscopes was detected between March 2020 and March 2023 in Tan Tock Seng Hospital Singapore.
Aim: To describe the use of whole-genome sequencing (WGS) in this investigation.
Methods: WGS was performed for all P.
Background: For Asian seabass (Lates calcarifer, Bloch 1790) cultured at sea cages various aquatic pathogens, complex environmental and stress factors are considered as leading causes of disease, causing tens of millions of dollars of annual economic losses. Over the years, we conducted farm-based challenges by exposing Asian seabass juveniles to complex natural environmental conditions. In one of these challenges, we collected a total of 1,250 fish classified as either 'sensitive' or 'robust' individuals during the 28-day observation period.
View Article and Find Full Text PDFPseudomonas aeruginosa ST308 clone has been reported to carry carbapenemase genes such as and but has been rarely associated with . A total of 199 P. aeruginosa ST308 clinical and environmental isolates obtained between April 2019 and November 2020 from a tertiary-care hospital in Singapore were characterized using whole-genome sequencing.
View Article and Find Full Text PDFArticle Synopsis
- Carbapenemase-producing Enterobacterales (CPE) infections in hospitals are thought to spread mainly from detected carriers to other patients in the same ward, but there is limited information on how plasmid-mediated transmission occurs at a larger scale.
- A study conducted in Singapore over 4.7 years analyzed 779 patients with CPE, finding that 42% of cases were due to clonal transmission, while 44.8% were linked to plasmid-mediated transmission.
- The results indicated that while direct contact in wards saw a decrease in transmission, indirect contacts (with no overlapping admission periods) remained high, highlighting the need for new strategies to tackle both clonal and plasmid-based infections in hospitals, as the latter
The convergence of carbapenem-resistance and hypervirulence genes in Klebsiella pneumoniae has led to the emergence of highly drug-resistant superbugs capable of causing invasive disease. We analyzed 556 carbapenem-resistant K. pneumoniae isolates from patients in Singapore hospitals during 2010-2015 and discovered 18 isolates from 7 patients also harbored hypervirulence features.
View Article and Find Full Text PDF