Thick filter neutrophil chemotaxis performed in the absence of albumin.

Migration through a filter is a technique widely used for the study of cell chemotaxis. Since the original description of the technique by Boyden in 1962 (J. Exp.

Influence of standard and novel LTB4 analogs on human neutrophil chemotaxis measured by the multiwell cap assay.

Standard and novel LTB4 analogs were tested for neutrophil chemoattractant activity using the multiwell cap assay (Evans et al. (1986) Biosc. Rep.

Comparison of LTB4- and C5a-stimulated chemotaxis of isolated human neutrophils: difference revealed by cell migration in thick filters using the multiwell cap procedure.

Under comparable conditions (90 min incubation in 2% albumin buffer) using 3 micron pore cellulose nitrate filters and the multiwell cap procedure (Evans et al., Bioscience Reports 6:1041, 1986), C5a was more potent than LTB4 as a chemoattractant (EC'50 s = 0.5 and 4 nM) and caused 5 times as many cells to completely traverse the filter.

Enhancement of N-formyl-methionyl-leucyl-phenylalanine (fMLP) binding to isolated human neutrophils.

Short chain aliphatic alcohols have previously been found to enhance fMLP binding to human neutrophils, presumably by non-specific mechanisms involving increased membrane hydrophobicity or decreased microviscosity. We report the discovery of highly potent fMLP binding enhancers with 30,000 times the potency of the alcohols. Activity of the compounds is specific since slight changes in structure drastically alter their ability to enhance binding and since closely related compounds inhibit rather than enhance binding.

Structure-activity profile of a series of novel triazoloquinazoline adenosine antagonists.

During a search for benzodiazepine receptor modulators, a highly potent adenosine antagonist (CGS 15943) was discovered. The compound was defined as a resonance-stabilized hybrid of the canonical structures 9-chloro-2-(2-furyl)[1,2,4]triazolo[1,5-c]quinazolin-5-amine (2a) and 9-chloro-2-(2-furyl)-5,6-dihydro[1,2,4]triazolo[1,5-c]-quinazolin- 5-imine (2b). Spectroscopic evidence and chemical reactivity in polar media favor the amine form 2a as the major contributor of the two canonical structures.