Effect of Trimethyltin Chloride on Slow Vacuolar (SV) Channels in Vacuoles from Red Beet (Beta vulgaris L.) Taproots.

In the present study, patch-clamp techniques have been used to investigate the effect of trimethyltin chloride (Met3SnCl) on the slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots. Activity of SV channels has been measured in whole-vacuole and cytosolic side-out patch configurations.

Effect of trimethyllead chloride on slowly activating (SV) channels in red beet (Beta vulgaris L.) taproots.

The patch-clamp technique was used to examine the effect of trimethyllead chloride (Met(3)PbCl) on SV channel activity in red beet (Beta vulgaris L.) taproot vacuoles. It was found that in the control bath the macroscopic currents showed the typical slow activation and a strong outward rectification of the steady-state currents.

The modified action of triphenyllead chloride on UVB-induced effects in albumin and lipids.

Previously we have shown a toxic effect of the organometallic compound triphenyllead (TPhPb) on cells. In the present study we evaluated the destructive effect of TPhPb on model systems--serum albumin and liposome membranes--alone and under UVB irradiation. UVB irradiation of bovine serum albumin results in protein S-S bond reduction, free SH- and CO- group formation and decrease in fluorescence intensity of tryptophans.

The location of organotins within the erythrocyte membrane in relation to their toxicity.

The aim of the present study on organotin compounds, which are toxic to biological systems, was to determine the relationship between the compounds' toxicity and their location in the lipid bilayer of the biological membrane. It was assumed that the degree of disturbance caused within the lipid bilayer of the membrane, which in turn depends on the depth of incorporation, was an appropriate measure of toxicity. Previous results from our studies on the effect of organotin chlorides on membranes, made by using infrared radiation and hemolysis of erythrocytes, indicated that tributyltin (TBT) is the most active in terms of its interaction with the erythrocyte membrane.

Superstatistics analysis of the ion current distribution function: Met3PbCl influence study.

A novel analysis of ion current time series is proposed. It is shown that higher (second, third and fourth) statistical moments of the ion current probability distribution function (PDF) can yield new information about ion channel properties. The method is illustrated on a two-state model where the PDF of the compound states are given by normal distributions.