Publications by authors named "S Pringle"
The oral cavity is a critical barrier with immunosurveillance capabilities. A detailed understanding of its cellular, molecular, and spatial architecture is essential for advancing precision medicine across aerodigestive tissues. Here, we present the first integrated atlas of human adult oral and craniofacial tissues, derived from single-cell RNA sequencing of ~250,000 cells from 70 samples across 13 niches, including salivary glands and oral mucosae.
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- Desorption electrospray ionization (DESI) tandem mass spectrometry (MS) is utilized to determine the mutation status of isocitrate dehydrogenase (IDH) in human gliomas, which have poor surgical outcomes due to their diffuse nature.* -
- IDH mutations are clinically significant, as they correlate with better prognoses; the mutant enzyme converts alpha-ketoglutaric acid to 2-hydroxyglutarate, leading to abnormal accumulation in cells.* -
- The study evaluates two mass spectrometry methods for their effectiveness in differentiating between IDH-mutant and IDH-wildtype samples, finding that the triple quadrupole has slightly better sensitivity, which could assist in
Background: While REIMS technology has successfully been demonstrated for the histological identification of ex-vivo breast tumor tissues, questions regarding the robustness of the approach and the possibility of tumor molecular diagnostics still remain unanswered. In the current study, we set out to determine whether it is possible to acquire cross-comparable REIMS datasets at multiple sites for the identification of breast tumors and subtypes.
Methods: A consortium of four sites with three of them having access to fresh surgical tissue samples performed tissue analysis using identical REIMS setups and protocols.