Upregulation of Urinary Alpha-2-HS Glycoprotein During Pregnancy of Murrah Buffaloes.

Urine samples were systematically collected from inseminated Murrah buffaloes (Bubalus bubalis) on days 0, 7, 14, 21 and 28 (with day 0 representing the day of artificial insemination). Following confirmation of pregnancy via trans rectal palpation 45 days of insemination, the animals were categorised into pregnant and non-pregnant groups (n = 10 each). The urine samples on 0, 7, 14, 21 and 28 days of pregnant and one sample from non-pregnant preferably collected on 28th day was used for SDS-PAGE after diafiltration.