Infantile achalasia cardia: A rare case report of a 3-month-old female with recurrent pneumonia successfully treated with surgical intervention.

Introduction: Achalasia, a rare esophageal disorder with an annual incidence of 0.11 per 100,000 in children, is characterized by impaired lower esophageal sphincter (LES) relaxation and peristalsis. Infantile cases are extremely uncommon and often linked to genetic conditions like Allgrove and Down syndrome.

Upper Urinary Tract Stereotactic Body Radiotherapy Using a 1.5 Tesla Magnetic Resonance Imaging-Guided Linear Accelerator: Workflow and Physics Considerations.

Advancements in radiotherapy technology now enable the delivery of ablative doses to targets in the upper urinary tract, including primary renal cell carcinoma (RCC) or upper tract urothelial carcinomas (UTUC), and secondary involvement by other histologies. Magnetic resonance imaging-guided linear accelerators (MR-Linacs) have shown promise to further improve the precision and adaptability of stereotactic body radiotherapy (SBRT). This single-institution retrospective study analyzed 34 patients (31 with upper urinary tract non-metastatic primaries [RCC or UTUC] and 3 with metastases of non-genitourinary histology) who received SBRT from August 2020 through September 2024 using a 1.

Effects of extremely low-frequency (50 Hz) electromagnetic fields on vital organs of adult Wistar rats and viability of mouse fibroblast cells.

In recent years, scientific communities have been concerned about the potential health effects of periodic electromagnetic field exposure (≤1 h/d). The objective of our study is to determine the impact of extremely low-frequency pulsed electromagnetic fields (ELF-PEMF) (1-3 mT, 50 Hz) on mouse fibroblast (red fluorescent protein (RFP)-L929) cells and adult Wistar rats to gain a comprehensive understanding of biological effects. We observed that RFP-L929 exhibits no significant changes in cell proliferation and morphology but mild elevation in aspartate aminotransferases, alanine aminotransferases, total bilirubin, serum creatinine, and creatine kinase-myocardial band levels in ELF-PEMF exposed groups under in vitro and in vivo conditions.

CRISPR-Cas-assisted phage engineering for personalized antibacterial treatments.

Background: In the age of modern medicine, CRISPR-Cas system-aided phage engineering has emerged as a major game changer for developing personalized antibacterial treatments. Modifying genomic DNA at a specific location leads to the inactivation of target genes, the acquisition of novel genetic features, and the correction of lethal gene mutations. Phages can be modified to precisely detect and control bacteria because of the vast possibilities of CRISPR-Cas-based genetic engineering.

Reverse Vaccinology and Immunoinformatics Strategy to Screen Oncogenic Proteins and Development of a Multiepitope Peptide Vaccine Targeting Protein Kinases against Oral Cancer: An in-silico Study.

Background: Despite standard therapies and immunotherapies, the mortality rate of patients with oral cancer remains high. Therefore, there is a need for more effective and targeted treatments. Multi-epitope vaccines have been developed for various cancers owing to their easy protection and delivery.