Publications by authors named "S Powles"

Background: Chlorsulfuron resistance and genetic dominance was evaluated in Raphanus raphanistrum genotypes homozygous (122-RR, 376-RR), heterozygous (122-RS, 376-RS) and compound heterozygous (122-R/376-R) for the target-site resistance mutations Ala-122-Tyr and Asp-376-Glu in the AHAS (acetohydroxyacid synthase) gene.

Results: At the AHAS level, 122-RR and 122-RS plants exhibited significantly higher I values than 376-RR and 376-RS plants, respectively. However, plants of the compound heterozygous genotype (122-R/376-R), showed no difference in AHAS activity compared to the 122-RS genotype but lower activity than the 122-RR genotype, and showed a nearly 400-fold greater I value than both genotypes (376-RR and 376-RS) carrying the 376-Glu allele.

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Article Synopsis
  • * In the study, specific genes (one dioxygenase and two P450 genes) were found to be more highly expressed in R plants compared to susceptible ones, contributing to herbicide resistance.
  • * Overexpressing these genes led to lower levels of mesotrione in plants and also provided resistance to other herbicides, suggesting that multiple gene overexpression may boost overall herbicide resistance in this raddish population.
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Background: We have previously demonstrated that an aldo-keto reductase (AKR) from Echinochloa colona (EcAKR4-1) can metabolize glyphosate and confers glyphosate resistance. This study aims to investigate if the EcAKR4-1 orthologs from Lolium rigidum also play a role in glyphosate resistance in non-target-site based, glyphosate-resistant (R) L. rigidum populations from Western Australia.

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Background: Urinary and faecal metabolic profiling have been extensively studied in gastrointestinal diseases as potential diagnostic markers, and to enhance our understanding of the intestinal microbiome in the pathogenesis these conditions. The impact of bowel cleansing on the microbiome has been investigated in several studies, but limited to just one study on the faecal metabolome.

Aim: To compare the effects of bowel cleansing on the composition of the faecal microbiome, and the urine and faecal metabolome.

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