A bread-based lyophilized C-strain CSF virus vaccine as an oral vaccine in pigs.

A live attenuated cereal-based classical swine fever (CSF) vaccine for oral application, a form of vaccine that farmers can use themselves, demonstrated the improvement of CSF control in backyard production systems in endemic areas. Due to the dependency on very low storage temperature (-20°C) of the cereal-based oral CSF vaccine, a lyophilized cereal-based oral vaccine has been developed and tested. Although some studies showed total protection against a virulent virus strain, the production procedure is still considered complex.

In vitro Development of Cystoisospora suis in Swine Kidney Cell Line, SK-6.

Cystoisospora suis (syn. Isospora suis) is an apicomplexan parasite which causes an important piglet coccidiosis resulting in commercial losses worldwide. Successful coccidiosis control using chemoprevention and chemotherapy as the recommended approach, nonetheless, conduces the extensive use of anticoccidial drugs that may lead to the emergence of drug-resistant parasites.

In vitro study of anti-coccidial activity of essential oils from indigenous plants against Eimeria tenella.

This study was designed to evaluate the in vitro anticoccidial properties against Eimeria tenella of different essential oils and their major active components. Efficacy of ten essential oils from different Thai indigenous plants were preliminarily screened and only those with potential were further tested for effective concentrations and identifying their active compounds. Oocysticidal property was evaluated in term of sporulation inhibition of oocysts and the percentage of unsporulated, sporulated and degenerated oocysts, after treatment with 125μg/ml of the selected essential oil, the sample was enumerated by haemocytometer, while coccidiocidal activity was assessed by the inhibition of sporozoite invasion in MDBK cell lines.

RNA interference targeting nucleocapsid protein (C) inhibits classical swine fever virus replication in SK-6 cells.

The application of RNA interference (RNAi) strategy for controlling classical swine fever could become a promising alternative to the conventional eradication measures, as it was recently shown for foot-and-mouth disease (Chen et al., 2004), influenza (Ge et al., 2003), porcine reproductive and respiratory syndrome (He et al.

Are low-density granulocytes the major target cells of classical swine fever virus in the peripheral blood?

The target cells of classical swine fever (CSF) virus in the peripheral blood of pigs infected with recent field isolates from Germany were studied. Eight weaned pigs were inoculated oronasally with the CSF virus field isolate Visbek/Han 95 and three weaners were inoculated with the isolate Losten/Freese 98. All pigs showed severe clinical signs typical of CSF and died or had to be euthanized between 9 and 24 days post-infection (dpi).