Postnatal neosporosis in dairy cattle in northeast Thailand.

A total of 83 dairy cows in Loei Province (Muang) and Nong Bua Lamphu (NBL) Province, northeast Thailand were sampled three times within 6 months in 1998 and their sera were examined for antibodies to Neospora caninum at a dilution of 1:100 in the indirect fluorescent antibody test (IFAT). In Muang the seroprevalence of N. caninum was 37.

Influence of Trypanosoma evansi infection on milk yield of dairy cattle in northeast Thailand.

Effect of subclinical Trypanosoma evansi infection on the milk yield of newly introduced Holstein Friesian dairy cattle were investigated. Five hundred pregnant heifers were introduced in Loei Province, northeast Thailand and a total of 168 blood samples were collected at 20 farms during 6 visits over 2 years. Trypanosomes were found in cattle in June and November 1996, after which the parasite was rarely seen.

Seroprevalence of antibodies to Neospora caninum and associated abortion in dairy cattle from central Thailand.

A total of 904 sera from dairy cattle in 11 provinces of central Thailand were tested for antibodies to Neospora caninum employing the indirect fluorescent antibody test (IFAT). Fifty four (6%) cattle were positive in IFAT, titres of 1:200 (16 cattle), 1:400 (9 cattle), 1:800 (14 cattle), 1:1600 (7 cattle), 1:3200 (6 cattle) and two positives. No significant difference was observed among the provinces.

Haemoparasite infections in newly introduced dairy cattle in Loei Province, Thailand: Trypanosoma evansi antigen levels by ELISA referring to abortion.

An antigen-detection enzyme-linked immunosorbent assay (Ag-ELISA) based on affinity-purified polyclonal antibody was utilised as an immunodiagnosis for Trypanosoma evansi infections in cattle. Five hundred pregnant heifers were introduced in Loei province, Thailand and a total of 271 samples were collected at 37 farms during four visits over a year commencing a month after the introduction. Each time the haematocrit centrifuge technique (HCT) was carried out as a field diagnosis for T.