Experimental Neurogenesis in the Embryos of the Gecko Paroedura picta.

This research introduces innovative tools for studying embryonic neurogenesis in the gecko Paroedura picta. Traditional research methods have been adapted for the gecko's unique biology, including variations of birthdating techniques and the implementation of EdU for tracking neuron generation. We also employ hybridization chain reaction (HCR) to detect specific mRNAs without the need for species-specific antibodies, providing a powerful and flexible tool for studying gene expression patterns in the gecko embryonic brain.

A tracking and monitoring system for transplanted organs during the transport phase.

Modern medicine has recognized organ and tissue transplantation as one of the most outstanding and promising advances. However, the demand for tissues and organs to be transplanted far exceeds the available supply. Due to this growing need, the problems inherent to the transplantation process have been pointed out in recent years.

Single-cell multiome and spatial profiling reveals pancreas cell type-specific gene regulatory programs driving type 1 diabetes progression.

Cell type-specific regulatory programs that drive type 1 diabetes (T1D) in the pancreas are poorly understood. Here we performed single nucleus multiomics and spatial transcriptomics in up to 32 non-diabetic (ND), autoantibody-positive (AAB+), and T1D pancreas donors. Genomic profiles from 853,005 cells mapped to 12 pancreatic cell types, including multiple exocrine sub-types.