Modulation of metabolism of Clostridium acetobutylicum grown in chemostat culture in a three-electrode potentiostatic system with methyl viologen as electron carrier.

The metabolism of Clostridium acetobutylicum was manipulated in chemostat culture at pH 5 and 6.5 in a three-electrode potentiostatic system with methyl viologen (MV) as the electron carrier. When a constant potential was applied at pH 5, the broth redox potential continuously decreased and, simultaneously, a high increase in the reduced MV concentration (MV(+.

Modulation of Carbon and Electron Flow in Clostridium acetobutylicum by Iron Limitation and Methyl Viologen Addition.

The metabolic flexibility of Clostridium acetobutylicum during growth on glucose with methyl viologen addition (1 mM) and/or iron limitation was examined in batch cultures at pH 5.5. The physiological effects of iron limitation and methyl viologen addition are additive, suggesting that they have different and complementary sites of action.