Expanded Substrate Specificity in D-Amino Acid Transaminases: A Case Study of Transaminase from .

Enzymes with expanded substrate specificity are good starting points for the design of biocatalysts for target reactions. However, the structural basis of the expanded substrate specificity is still elusive, especially in the superfamily of pyridoxal-5'-phosphate-dependent transaminases, which are characterized by a conserved organization of both the active site and functional dimer. Here, we analyze the structure-function relationships in a non-canonical D-amino acid transaminase from , which is active towards D-amino acids and primary ()-amines.

In search for structural targets for engineering d-amino acid transaminase: modulation of pH optimum and substrate specificity.

The development of biocatalysts requires reorganization of the enzyme's active site to facilitate the productive binding of the target substrate and improve turnover number at desired conditions. Pyridoxal-5'-phosphate (PLP) - dependent transaminases are highly efficient biocatalysts for asymmetric amination of ketones and keto acids. However, transaminases, being stereoselective enzymes, have a narrow substrate specificity due to the ordered structure of the active site and work only in neutral-alkaline media.

Alginate/chitosan hydrogels as perspective transport systems for cefotaxime.

This work reports synthesis of pH-responsive alginate/chitosan hydrogel spheres with the average diameter of 2.0 ± 0.05 mm, which contain cefotaxime that is an antibiotic of the cefalosporine group.

To the Understanding of Catalysis by D-Amino Acid Transaminases: A Case Study of the Enzyme from .

Pyridoxal-5'-phosphate (PLP)-dependent transaminases are highly efficient biocatalysts for stereoselective amination. D-amino acid transaminases can catalyze stereoselective transamination producing optically pure D-amino acids. The knowledge of substrate binding mode and substrate differentiation mechanism in D-amino acid transaminases comes down to the analysis of the transaminase from .

[The regional characteristics of endocrine pathology in the Perm kraii].

Nowadays, focus of research is shifted to epidemiology of non-communicable diseases. Hence, actuality of study purpose to investigate epidemiological characteristics of endocrine diseases. The data of population appealability to medical organizations in the Perm region during last 20 years was used.