Publications by authors named "S P Oakley"
Objectives: The determinants of rheumatoid arthritis (RA) severity and excess cardiovascular disease (CVD) are incompletely understood. Biomechanical factors are known to influence RA severity. Articular stiffness correlates with arterial and skin stiffness.
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- Whole-transcriptome RNA sequencing using Oxford Nanopore Technologies shows potential for analyzing gene expression in pathogenic bacteria, including antimicrobial resistance genes.
- The study assessed the direct cDNA and PCR-cDNA sequencing kits by analyzing four bacterial isolates from bloodstream infections, utilizing various techniques to minimize bias.
- Results indicated that the PCR-cDNA kit provided higher yield, but users should be cautious of potential bias in genes with very high or low GC content.
Article Synopsis
- The study showcases a new technology, Adaptive Channel Bacterial Capture (ACBC), which effectively captures, enriches, and identifies bacterial pathogens from low-density samples with nearly perfect efficiency.
- This method uses simple fabrication techniques and can capture various bacteria, including common pathogens, even when present in concentrations below 1000 cells per mL.
- The ACBC device can also quickly determine antibiotic susceptibility of identified bacteria using fluorescence imaging and machine learning techniques, combining bacterial capture and rapid testing in a single tool.
Antibiotic resistance is an urgent global health challenge, necessitating rapid diagnostic tools to combat its threat. This study uses citizen science and image feature analysis to profile the cellular features associated with antibiotic resistance in Escherichia coli. Between February and April 2023, we conducted the Infection Inspection project, in which 5273 volunteers made 1,045,199 classifications of single-cell images from five E.
View Article and Find Full Text PDFAims: To explore levers and barriers to providing culturally responsive care for general practice nurses (GPNs) using normalization process theory.
Design: A self-administered online cross-sectional survey.
Methods: A participatory co-designed adapted version of the normalization of complex interventions measure (NoMAD) validated tool was distributed to a convenience sample of GPNs between December 2022 and February 2023.