In vitro study of a siRNA delivery liposome constructed with an ionizable cationic lipid.

Objectives: Small interfering RNA (siRNA) can silence disease-related genes through sequence-specific RNA interference (RNAi). Cationic lipid-based liposomes effectively deliver nucleic acids into the cytoplasm but often exhibit significant toxicity. This study aims to synthesize a novel ionizable lipid, Nε-laruoyl-lysine amide (LKA), from natural amino acids, constructed LKA-based liposomes, and perform physicochemical characterization and cell-based experiments to systematically evaluate the potential of these ionizable lipid-based liposomes for nucleic acid delivery.

Impact of silicone midline catheter tip location on catheter-related complications and indwelling duration: results from a multicentre randomised clinical trial in China.

Objective: To explore the impact of the terminal tip location of silicone midline catheter (MC, a type of intravenous catheter measuring 20-30 cm in length and inserted into upper arm veins using a modified Seldinger technique) in the subclavian vein group versus axillary vein group on catheter-related complications and indwelling duration.

Design: This is a randomised controlled study.

Setting: Twenty-seven tertiary hospitals in China.

High-quality Population-specific Haplotype-resolved Reference Panel in the Genomic and Pangenomic Eras.

Large-scale international and regional human genomic and pangenomic resources derived from population-scale biobanks and ancient DNA sequences have provided significant insights into human evolution and the genetic determinants of complex diseases and traits. Despite these advances, challenges persist in optimizing the integration of phasing tools, merging haplotype reference panels (HRPs), developing imputation algorithms, and fully exploiting the diverse applications of post-imputation data. This review comprehensively summarizes the advancements, applications, limitations, and future directions of HRPs in human genomics research.

Association of obstructive sleep apnea with cardiovascular events in acute coronary syndrome patients with dual risk of remnant cholesterol and low-grade inflammation: a post-hoc analysis of the OSA-ACS study.

Purpose: Obstructive sleep apnea (OSA) is a recognized cardiovascular risk factor, yet the benefits of intervention remain uncertain due to the heterogeneity among OSA patients. We aimed to explore the association of OSA with cardiovascular outcomes in acute coronary syndrome (ACS) patients with dual risk of elevated remnant cholesterol (RC) and low-grade inflammation indicated by high-sensitivity C-reactive protein (hs-CRP).

Methods: This study is a post-hoc analysis of OSA-ACS project enrolled 1833 ACS patients from January 2015 to December 2019, who underwent a sleep study, categorized into four groups by median levels of RC and hs-CRP: RC and low-grade inflammation risk (RCIR), low-grade inflammation risk (LDIR), RC risk (RCR), and no residual risk.

Successful Treatment of Eosinophilic Pustular Folliculitis With Dupilumab in a 9-Year-Old Boy.

A 9-year-old boy with eosinophilic pustular folliculitis (EPF) was successfully treated with dupilumab. While reported in adult EPF, this is the first case of EPF treated with dupilumab in a child. Our case suggests dupilumab might be a favorable therapeutic option for EPF in children.