[Dot immunoassay with colloidal silver particles as a marker of specific antigen for testing human sera for brucellosis].

Whether the dot immunoassay is suitable for the detection of Brucella antibodies in human sera by using a colloidal silver-labeled Brucella specific antigen as a diagnostic tool is assessed. The antigen was the B. abortus 19BA protein polysaccharide complex isolated by Brucella acetic acid hydrolysis.

[Evaluation of vaccinal immunogenesis by the peroxidase method].

The possibility of evaluating functional immunomorphogenesis in the course of the vaccinal process after the injection of conjugated and live brucellosis vaccines, as well as conjugated plague antigen and Yersinia pestis strain EV, to guinea pigs has been shown by means of the direct and two-layer variants of the immunoperoxidase assay. The dynamics of the accumulation of globulin-producing cells in the immunocompetent organs and the time course of immunoglobulin titers in the peripheral blood after the injection of live and conjugated vaccines have been followed up. These data may be used for the morphological evaluation of approved preparations.

[Glucose metabolism in Yersinia enterocolitica cells].

The presence of hexokinase, aldolase, glyceraldehyde-phosphate dehydrogenase, phosphoglycerate kinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase has been detected in Y. enterocolitica, which indicates the glycolytic and pentosophosphate pathways of glucose catabolism. Y.