Low-molecular-weight thiol transferases in redox regulation and antioxidant defence.

Low-molecular-weight (LMW) thiols are produced in all living cells in different forms and concentrations. Glutathione (GSH), coenzyme A (CoA), bacillithiol (BSH), mycothiol (MSH), ergothioneine (ET) and trypanothione T(SH) are the main LMW thiols in eukaryotes and prokaryotes. LMW thiols serve as electron donors for thiol-dependent enzymes in redox-mediated metabolic and signaling processes, protect cellular macromolecules from oxidative and xenobiotic stress, and participate in the reduction of oxidative modifications.

Mass Spectral Library Methods for Analysis of Site-Specific N-Glycosylation: Application to Human Milk Proteins.

We present a mass spectral library-based method for analyzing site-specific N-linked protein glycosylation. Its operation and utility are illustrated by applying it to both newly measured and available proteomics data of human milk glycoproteins. It generates two varieties of mass spectral libraries.

MS_Piano: A Software Tool for Annotating Peaks in CID Tandem Mass Spectra of Peptides and N-Glycopeptides.

Annotating product ion peaks in tandem mass spectra is essential for evaluating spectral quality and validating peptide identification. This task is more complex for glycopeptides and is crucial for the confident determination of glycosylation sites in glycoproteins. MS_Piano (ass pectrum eptde ntation) software was developed for reliable annotation of peaks in collision induced dissociation (CID) tandem mass spectra of peptides or N-glycopeptides for given peptide sequences, charge states, and optional modifications.

Scaling up and scaling down supply chains in volatile resource-based economies.

The growth of mobile workforces to support diversified resource extraction activities, compared to historically single-industry towns, represents a key change in rural and remote resource landscapes that has accelerated since the 1980s. Mobile workforces can present many opportunities to rural communities and economies. However, the capacity, viability and competitiveness of rural-based businesses to engage in supply chains serving mobile labour may be undermined by limited attention to how businesses manoeuvre downturns while maintaining a level of readiness to recover and scale-up in order to meet emerging mobile workforce needs.