RNA Modifications and Prp24 Coordinate Lsm2-8 Binding Dynamics during U6 snRNP Assembly.

In eukaryotes, the process of intron removal from nuclear pre-mRNA is performed by the spliceosome, a dynamic molecular machine composed of small nuclear ribonucleoproteins (snRNPs; U1, U2, U4, U5, and U6) and dozens of other protein splicing factors. The U6 snRNP contains the U6 snRNA and the proteins Prp24 and Lsm2-8 heteroheptamer. A key feature of the snRNP is a modified U6 snRNA 3' end, which in (yeast) contains a 3' phosphate.

A novel L-RNA aptamer to regulate the pUG fold RNA-induced gene expression in vivo.

G-quadruplex (G4) is a guanine-rich secondary structure found in DNA and RNA involved in various biological roles. Recently, a non-canonical RNA G-quadruplex (rG4), known as poly(UG) (pUG) fold, was discovered in Caenorhabditis elegans. This unique structure was found to induce RNA interference (RNAi) upon recruitment of RNA-dependent RNA polymerase (RdRP), resulting in trans-generational gene silencing.

The Reversibility of Cardiac Damage After Transcatheter Aortic Valve Implantation and Short-Term Outcomes in a Real-World Setting.

Aims: This study aims to assess the changes in cardiac damage stage in a real-world cohort of patients undergoing transcatheter aortic valve implantation (TAVI) and to investigate the prognostic value of cardiac damage stage evolution.

Methods And Results: Patients with severe AS undergoing TAVI were retrospectively analyzed. A 5-stage system based on the presence and extent of cardiac damage assessed by echocardiography was applied before and 6 months after TAVI.

Conceptualizing a load and volume autoregulation integrated velocity model to minimize neuromuscular fatigue and maximize neuromuscular adaptations in resistance training.

Resistance training (RT) load and volume are considered crucial variables to appropriately prescribe and manage for eliciting the targeted acute responses (i.e., minimizing neuromuscular fatigue) and chronic adaptations (i.

What are the experiences of Aboriginal and/or Torres Strait Islander midwifery students and midwives? A scoping review.

Background: Aboriginal and/or Torres Strait Islander midwives are underrepresented within the midwifery workforce and is likely compounded by lower graduate rates. This review is a part of the Midwifery Futures Project. It explores the experiences of Aboriginal and/or Torres Strait Islander midwives and midwifery students to illuminate issues impacting work and study and uncover successful strategies towards addressing current disparities.