Plasmodiophora brassicae affects host gene expression by secreting the transcription factor-type effector PbZFE1.

The protist pathogen Plasmodiophora brassicae hijacks the metabolism and development of host cruciferous plants and induces clubroot formation, but little is known about its regulatory mechanisms. Previously, the Pnit2int2 sequence, a sequence around the second intron of the nitrilase gene (BrNIT2) involved in auxin biosynthesis in Brassica rapa ssp. pekinensis, was identified as a specific promoter activated during clubroot formation.

Antiproliferative activity of protocatechualdehyde on Chinese hamster cells grown in culture.

The mode of cytotoxic action of protocatechualdehyde on V79 cells was examined. The cytotoxicity of protocatechualdehyde on V79 cells was time- and cell cycle-dependent. The lethal action is selective for the cells at S phase only.

Cytomorphological study of cultured mammalian cells treated with benzaldehyde, catechol and their derivatives with special reference to inhibition of DNA synthesis.

Cytotoxicity and cytomorphological changes induced in cultured mammalian cells by the application of benzaldehyde, pyrocatechol and their derivatives were examined. Among the compounds tested, 3,4-dihydroxybenzaldehyde (protocatechualdehyde) and 1,2-dihydroxybenzene (pyrocatechol) produced cytomorphological changes characteristic of specific inhibitors of DNA synthesis in V79 cells and EJ-1 cells. However, they did not induce such changes in HeLa cells.