Publications by authors named "S O Onigbinde"
Introduction: The identification and characterization of glycopeptides through LC-MS/MS and advanced enrichment techniques are crucial for advancing clinical glycoproteomics, significantly impacting the discovery of disease biomarkers and therapeutic targets. Despite progress in enrichment methods like Lectin Affinity Chromatography (LAC), Hydrophilic Interaction Liquid Chromatography (HILIC), and Electrostatic Repulsion Hydrophilic Interaction Chromatography (ERLIC), issues with specificity, efficiency, and scalability remain, impeding thorough analysis of complex glycosylation patterns crucial for disease understanding.
Areas Covered: This review explores the current challenges and innovative solutions in glycopeptide enrichment and mass spectrometry analysis, highlighting the importance of novel materials and computational advances for improving sensitivity and specificity.
Article Synopsis
- Glyphosate, a common herbicide since the 1970s, has raised health concerns despite initial beliefs of its safety due to the absence of a specific biological pathway in humans and animals.
- The study investigated changes in -glycosylation, a post-translational modification of proteins, in rats exposed to glyphosate-based herbicides, utilizing advanced techniques like LC-MS/MS for analysis.
- Findings indicated significant alterations in the -glycan profile of rat serum, with male rats showing more susceptibility to changes than females, suggesting potential neurological implications and enhancing understanding of glyphosate's toxic effects.
Introduction: Glyphosate-based herbicides (GBHs) have been shown to have significant neurotoxic effects, affecting both the structure and function of the brain, and potentially contributing to the development of neurodegenerative disorders. Despite the known importance of glycosylation in disease progression, the glycome profile of systems exposed to GBH has not been thoroughly investigated.
Methods: In this study, we conducted a comprehensive glycomic profiling using LC-MS/MS, on the hippocampus and prefrontal cortex (PFC) of juvenile rats exposed to GBH orally, aiming to identify glyco-signature aberrations after herbicide exposure.
Background: The study of glycopeptides is associated with challenges regarding the microheterogeneity of different isomeric glycans occupying the same glycosylation sites in glycoproteins. It is immensely valuable to perform both qualitative and quantitative site-specific glycosylation analysis of glycopeptide isomers due to their link to several diseases. Achieving isomeric separation of glycopeptides is particularly challenging due to the low abundance of glycopeptides as well as inefficient ionization.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates shoulder pain and MRI findings in adult Nigerians, noting the scarcity of data on this demographic.
- Over 100 adult patients were analyzed, revealing that common conditions include supraspinatus tendinopathy, acromioclavicular joint arthropathy, and subacromial-subdeltoid bursitis.
- Findings suggest that shoulder abnormalities are prevalent regardless of gender, with specific pathologies highlighted as significant among participants.