A signaling inspired synthetic toolkit for efficient production of tyrosine phosphorylated proteins.

Tyrosine phosphorylation is an important post-translational modification that regulates many biochemical signaling networks in multicellular organisms. To date, 46,000 tyrosines have been observed in human proteins, but relatively little is known about the function and regulation of most of these sites. A major challenge has been producing recombinant phospho-proteins in order to test the effects of phosphorylation.

Striving to establish patient participation in rehabilitation: the challenges experienced by nursing staff when changing practice to include the patient's perspective.

Purpose: Patient participation is a complex issue and difficult to establish, but essential to successful spinal cord injury rehabilitation. The purpose of this study was to explore the challenges experienced by nursing staff when they wanted to include the patient's perspective in their rehabilitation.

Methods: Action research methodology was applied to increase knowledge, develop competences, and ultimately change practice.

A bioinformatic approach for the prediction and functional classification of Toxoplasma gondii long non-coding RNAs.

Long non-coding RNAs (lncRNAs) have emerged as significant players in diverse cellular processes, including cell differentiation. Advancements in computational methodologies have facilitated the prediction of lncRNA functions, enabling insights even in non-model organisms like pathogenic parasites, in roles such as parasite development, antigenic variation, and epigenetics. In this work, we focus on the apicomplexan Toxoplasma gondii differentiation process, where the infective stage, tachyzoite, can develop into the cysted stage, bradyzoite, under stress conditions.