THE EFFECT OF INTIMACY AND STATUS DISCREPANCY ON SALIENT AND NON-SALIENT CONFLICT STRATEGIES OF JAPANESE.

It has been claimed that Japanese people prefer passive forms of conflict strategies to preserve interpersonal harmony. This study aimed to identify some conditions in which such passive strategies are used. The effects of target intimacy and status discrepancy on the intent and use of salient and non-salient conflict strategies were examined, along with respondent sex differences.

Cytotoxic and mutagenic effects of chronic low-dose-rate irradiation on TERT-immortalized human cells.

To analyze the genetic effects of low-dose-rate radiation on human cells, we used human telomere reverse transcriptase (TERT)-immortalized fibroblast cells obtained from normal individuals. We studied the effect of low-dose-rate (0.3 mGy/ min) and high-dose-rate (2 Gy/min) radiation on cells in a confluent state.

Establishment of immortal normal and ataxia telangiectasia fibroblast cell lines by introduction of the hTERT gene.

To establish immortal human cells, we introduced the human catalytic subunit of telomerase (hTERT) gene into skin fibroblast cells obtained from normal and ataxia telangiectasia (AT) individuals of Japanese origin. After hTERT introduction, these cells continue to grow beyond a population doubling number of 200 while maintaining their original radiosensitivity. Inductions of p53, phosphorylation of Ser15 in p53, and induction of p21 by X-ray irradiation in immortal cells derived from normal individual were not affected by the hTERT introduction.

Absence of correlation of MUC1 expression to malignant behavior of renal cell carcinoma in experimental systems.

A correlation between MUC1 expression in renal cell carcinomas (RCCs) and the clinical stages was previously demonstrated. To assess whether MUC1 expression is causally related to malignant tumor behavior, MUC1 cDNA was stably transfected into a renal carcinoma cell line SN12C that expresses trace levels of MUC1. MUC1 with sialylated carbohydrate chains was detected on the surface of transfected cells in two independent experiments.

Intercept-PCR, an improvement for elevating performance to find a new member of a certain gene family.

We have established a method by which the performance of reverse transcriptase coupled polymerase chain reaction (RT-PCR) for seeking a new gene is improved. The actual procedure is quite easy: it is only to add several specific oligonucleotides into the reaction mixture of the usual RT-PCR. To verify the effectiveness of this method is also easy: it is only to detect the PCR products in the preliminary experiment.