[CELL-MOLECULAR BASIS OFNEUROIMMUNE INTERACTIONS DURING STRESS].

The review represents a modern concept about cells-molecular basis of mechanisms of neuro-immune interactions, the data on the effects of destabilizing factors (electric pain stimulation, rotation, cold and psychoemotional stress) on the functioning of neurons and immune cells. It must be underlined, that under the stress conditions take place the alterations of ligand-receptors interactions on the membrane of lymphocyte. In particular the reaction of these cells to regulating signal - application of Interleikin-1 grow up after mild stress, but it falls down after an influence of severe stress factors.

[Impairments of rats' behavioral reactions, concentration of corticosterone and expression of cytokine's gene under experimental postviral fatigue].

The aim of the main research was investigated of interaction of neural, endocrine and immune systems under experimental postviral fatigue, behavioral reactions, level of corticosterone, changes of IL-3 and IL-10 gene expression in rats' hypothalamus and INF-α in hypothalamus and spleen were analyzed. It has been shown the decrease of physical activity, increasing of corticosterone's level and gene expression of cytokines after injection of Poly I:C as a model of postviral fatigue. After remedication of Poly I:C increasing of physical activity was shown.

Stress-induced changes in the functional activity of the neuroendocrine system: the modulatory activity of derinat.

Changes in the functional activity of the hypothalamo-hypophyseal-adrenocortical system (HHACS) and hypothalamo-hypophyseal-gonadal system (HHGS) in conditions of different levels of stress and the possibility of correcting them using the native DNA formulation Derinat, which has immunomodulatory properties, were studied. The vectors of changes in hormonal reactions in both systems were found to be independent of the intensity of stress: there were increases in corticosterone levels and decreases in testosterone concentrations in different stress models. Intraperitoneal administration of Derinat at doses of 10 and 50 mg/kg increased HHACS and HHGS activity and reversed the stress-induced reduction in the blood testosterone concentration, which may be evidence that Derinat has stress-protecting effects.

[Cellular and molecular mechanisms of interaction between the neuroendocrine and immune systems under chronic fatigue syndrome in experiment].

One of the main mechanisms of chronic fatigue syndrome development involves disturbances of interaction between the immune and neuroendocrine systems. The adequate experimental model for the search of these mechanisms is induction of fatigue in animals via the single intraperitoneal administration of synthetic double-stranded RNA - Poly I : C. Investigation of alterations in cytotoxic and proliferation activities of splenocytcs, the intensity of immunomodulatory cytokines signaling via the sphingomyelin pathways in membrane P2 fraction of the brain cortex, as well as the activity of hypothalamic-pituitary adrenal (HP A) axis in the dynamics of chronic fatigue syndrome in rats has performed.

[Stress-induced changes in functional activity of the neuro-endocrine system: modulatory action of derinat].

Changes in functional activity of HPA and HPG axes under stress influences of different intensity, and possible ways for their correction by native DNA preparation: derinat, possessing immune modulator effects, were studied. It was shown that the vector of changes in hormone's reactions of both axes did not depend on the intensity of stress influences: different models of stress increased corticosterone level and decreased testosterone level in rats' blood. Intraperitoneal injection of 10 and 50 mg/kg BW doses of Derinat to rats enhanced HPA and HPG axes activity, reversed stress-induced decrease of testosterone concentration in blood, that may indicate a stress-protective effect of derinat.