Publications by authors named "S N Moskalenko"
Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants.
ACS Phys Chem Au
July 2024
Article Synopsis
- Genetically encoded voltage indicators (GEVIs), especially those based on archaerhodopsin-3, are useful for visualizing changes in cell membrane potential.
- To improve these tools, researchers aimed to create new GEVIs with better fluorescence and absorption properties.
- The study successfully enhanced the fluorescence of archaerhodopsin-3 by stabilizing specific protein conformations through targeted mutations, leading to the design of new variants with a broader absorption range and significantly brighter emissions.
Article Synopsis
- Yeast have two critical translation termination factors, eRF1 and eRF3, whose mutations lead to the amplification of their mutant alleles, which is vital for cell survival.
- The study used RNA-Seq and proteome analysis to explore how yeast cells adapt to nonsense mutations and found significant gene expression changes affecting the cell cycle.
- The researchers suggest that the adaptation involves a delay in cell cycle progression, particularly at the G2-M transition, resulting in extended S and G2 phases that promote the replication of the mutant alleles.
The gene encodes a cytosolic protein that binds to the signaling cascade component neuronal nitric oxide synthase (nNOS). It is associated with many different disorders, such as schizophrenia, post-traumatic stress disorder, autism, cardiovascular disorders, and breast cancer. The NOS1AP (also known as CAPON) protein mediates signaling within a complex which includes the NMDA receptor, PSD-95, and nNOS.
View Article and Find Full Text PDFThe review discusses the role that proteins interacting with the translation termination factors eRF1 and eRF3 play in the control of protein synthesis and prionization. These proteins interact not only with each other, but also with many other proteins involved in controlling the efficiency of translation termination, and associate translation termination with other cell processes. The termination of translation is directly related not only to translation re-initiation and ribosome recycling, but also to mRNA stability and protein quality control.
View Article and Find Full Text PDFArticle Synopsis
- Protein synthesis (translation) is essential for living cells and consists of three main steps: initiation, elongation, and termination, with specific factors like eRF1 and eRF3 involved in yeast termination.
- Deletion of the genes encoding these factors is lethal for yeast, but some strains with nonsense mutations can survive through a feedback mechanism related to readthrough of stop codons, though the details of this mechanism are still unclear.
- Whole-genome sequencing revealed no common genetic changes, but increased copy numbers of mutant alleles were observed, with qPCR confirming that gene amplification is a key adaptation strategy for yeast with nonsense mutations in release factor genes.