A systematic benchmark of Nanopore long-read RNA sequencing for transcript-level analysis in human cell lines.

The human genome contains instructions to transcribe more than 200,000 RNAs. However, many RNA transcripts are generated from the same gene, resulting in alternative isoforms that are highly similar and that remain difficult to quantify. To evaluate the ability to study RNA transcript expression, we profiled seven human cell lines with five different RNA-sequencing protocols, including short-read cDNA, Nanopore long-read direct RNA, amplification-free direct cDNA and PCR-amplified cDNA sequencing, and PacBio IsoSeq, with multiple spike-in controls, and additional transcriptome-wide N-methyladenosine profiling data.

Correction: Han et al. Genotypic Homogeneity in Distinctive Transforming Growth Factor-Beta-Induced (TGFBI) Protein Phenotypes. 2021, , 1230.

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ZnO-Embedded Carboxymethyl Cellulose Bioplastic Film Synthesized from Sugarcane Bagasse for Packaging Applications.

This research explores the synthesis of carboxymethyl cellulose (CMC) for the development of a cost-effective bioplastic film that can serve as a sustainable alternative to synthetic plastic. Replacing plastic packaging with CMC-based films offers a solution for mitigating environmental pollution, although the inherent hydrophilicity and low mechanical strength of CMC present significant challenges. To address these limitations, zinc oxide nanoparticles (ZnO NPs) were employed as a biocompatible and non-toxic reinforcement filler to improve CMC's properties.

Management of Dupuytren's Disease: A Multi-Centric Comparative Analysis Between Experienced Hand Surgeons Versus Artificial Intelligence.

: Dupuytren's fibroproliferative disease affecting the hand's palmar fascia leads to progressive finger contractures and functional limitations. Management of this condition relies heavily on the expertise of hand surgeons, who tailor interventions based on clinical assessment. With the growing interest in artificial intelligence (AI) in medical decision-making, this study aims to evaluate the feasibility of integrating AI into the clinical management of Dupuytren's disease by comparing AI-generated recommendations with those of expert hand surgeons.

The Oncological Outcome of Postoperative Radiotherapy in Patients with Node-Negative Early-Stage (T1/T2/N0) Oral Squamous Cell Carcinoma and Perineural Invasion: A Meta-Analysis.

To evaluate the impact of postoperative radiotherapy (PORT) on oncological outcomes in node-negative early-stage oral squamous cell carcinoma (OSCC) with perineural invasion (PNI). : A systematic review and meta-analysis was conducted using the PubMed, EMBASE, and Scopus databases for the period from 2000 to 2024. Studies comparing PORT versus observation in pN0 early-stage OSCC with PNI were included.