[Designing of the pp65 recombinant antigen of human cytomegalovirus and investigation of its immunochemical properties].

The primary and secondary structures of the pp65 phosphoprotein of human cytomegalovirus coded by the UL83 gene were studied by the methods of computer-aided analysis. An immunodominant protein fragment with 3 antigenic determinant was detected. The UL83 fragment coding the selected region was amplified and cloned in bacterial expressing vector.

[Designing of a liquid serum panel containing or not class IgG antibodies specific to human cytomegalovirus].

Designing of a liquid serum panel for the determination of class G antibodies specific to human cytomegalovirus (HCMV) is under discussion. Sera were selected by ELISA for antibodies to HCMV and by PCR for CMV DNA. The serum panel comprises samples of positive and negative sera with high and low titers.

[Evaluation of the immunological effectiveness of the planned vaccinal prophylaxis of diphtheria and tetanus in Sverdlovsk and Sverdlovsk Province].

The level and intensity of antitoxic immunity to diphtheria and tetanus in children and adolescents were determined. The presence of tetanus antitoxin in titers exceeding the protective level in 96.3-98.