[Serodiagnosis of chlamydiosis: "Chlamy-IgG-DS-Tr"-- the first domestic immunoenzyme recombinant test-system for determination of anti-Chlamydia trachomatis class G antibodies].

The first Native test-system for determination of class G antibodies to Chlamydia trachomatis with chlamydia recombinant antigen was elaborated Test-system efficacy was demonstrated in clinical trials. The sensibility, specific activity and suitability of the "Chlamy-IgG-DS-Tr" were the same as for import analogous.

The effect of pH on biological activity of plant cytotoxin cauloside C.

The effect of plant carboxyl-containing glycoside cauloside C upon eucaryotic cells has been studied. The glycoside interacts with cells as a pH-dependent cytotoxin and increases K+ leakage and Ca2+ uptake with strong action in acidic media Cell viability after glycoside action at acidic pH may be recovered by the shift of medium pH from 5.6 to 7.

[Isolation and characterization of immortalized human fibroblasts].

A clone of immortalized human fibroblasts with an extended life span in culture was obtained after infection with a temperature-sensitive mutant (tsA 239) of SV40. In comparison with the parental cells, the clone exhibited an increase in the efficiency of plating, decreased doubling time, and serum dependence. No anchorage independence was observed.

[The use of nested polymerase chain reaction method for rapid detection of cytomegalovirus in peripheral blood leukocytes of patients after bone marrow transplantation].

Nested polymerase chain reaction was used for rapid detection of human cytomegalovirus in blood samples of patients subjected to bone marrow transplantation. Use of the four-primer system appreciably improved the sensitivity and specificity of the method and helped eliminate the difficulties arising in the detection of various viral strains with the minor nucleotide substitutes in the DNA template. The proposed method is a reliable tool for the early diagnosis of cytomegaloviral viremia and for monitoring the efficacy of antiviral therapy.