Amitriptyline Attenuates Ca Responses Induced by Glutoxim and Molixan in Macrophages.

Using Fura-2AM microfluorimetry, we have shown for the first time that sigma-1 receptor agonist, tricyclic antidepressant amitriptyline, significantly inhibits glutoxim- and molixan-induced Ca-responses in rat peritoneal macrophages. The results suggest possible involvement of sigma-1 receptors in the signaling cascade induced by glutoxim or molixan and leading to intracellular Ca concentration increase in macrophages.

Sigma-1 Receptor Antagonist Haloperidol Attenuates Store-Dependent Ca Entry in Macrophages.

Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with sigma-1 receptor antagonist haloperidol leads to a significant inhibition of the store-dependent Ca entry induced by endoplasmic Ca-ATPase inhibitors thapsigargin or cyclopiazonic acid in rat peritoneal macrophages. The results suggest the involvement of the sigma-1 receptor in the regulation of storedependent Ca entry in macrophages.

Trifluoperazine Attenuates Store-Dependent Ca Entry in Macrophages.

Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with the calsequestrin inhibitor neuroleptic trifluoperazine leads to a significant inhibition of the store-dependent Ca entry induced by endoplasmic Ca-ATPase inhibitors thapsigargin or cyclopiazonic acid in rat peritoneal macrophages. The results suggest calsequestrin involvement in the regulation of the store-dependent Ca entry in macrophages.

Phospholipase A Inhibitors Modulate the Effect of Trifluoperazine on the Intracellular Ca Concentration in Macrophages.

Using Fura-2AM microfluorimetry, it was shown for the first time that phospholipase A inhibitors 4-bromophenacyl bromide and glucocorticosteroids prednisolone and dexamethasone attenuate Ca responses induced by neuroleptic trifluoperazine in macrophages. The results suggest the involvement of phospholipase A and arachidonic acid metabolism cascade in the effect of trifluoperazine on intracellular Ca concentration in macrophages.

The effect of chlorpromazine on intracellular Ca concentration in macrophages.

Using Fura-2AM microfluorimetry, it was shown for the first time that neuroleptic chlorpromazine causes intracellular Ca concentration increase in macrophages due to Ca mobilization from intracellular Ca stores and subsequent Ca entry from the external medium. Chlorpromazine-induced Ca entry is inhibited by La and 2-aminoethoxydiphenyl borate and is associated with Ca store depletion.