Publications by authors named "S Munirah"
Article Synopsis
- This study explores how human adipose-derived stem cells (ASC) can differentiate into bone-forming cells when co-cultured with human osteoblasts (HOB) on specific scaffolds made from polycaprolactone (PCL) and hydroxyapatite (HA).
- Using different ratios of ASC and HOB in the co-culture, researchers assessed their growth and bone formation abilities through various tests including cell morphology, proliferation rates, and gene expression.
- Results indicated that a 2:1 ratio of ASC to HOB on PCL-HA scaffolds led to the best outcomes in terms of bone-related activity and gene expression, suggesting that this approach could effectively support early bone development.
The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)-3-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups.
View Article and Find Full Text PDFMonolayer culture expansion remains as a fundamental step to acquire sufficient number of cells for 3D constructs formation. It has been well-documented that cell expansion is however accompanied by cellular dedifferentiation. In order to promote cell growth and circumvent cellular dedifferentiation, we evaluated the effects of Transforming Growth Factor Beta-2 (TGF-β2), Insulin-like Growth Factor-I (IGF-I) and basic Fibroblast Growth Factor (bFGF) combination on articular chondrocytes culture and 'chondrocytes-fibrin' construct formation.
View Article and Find Full Text PDFChondrocytes were isolated from articular cartilage biopsy and were cultivated in vitro. Approximately 30 million of cultured chondrocytes per ml were incorporated with autologous plasma-derived fibrin to form three-dimensional construct. Full-thickness punch hole defects were created in lateral and medial femoral condyles.
View Article and Find Full Text PDFAutologous versus pooled human serum for articular chondrocyte growth.
J Orthop Surg (Hong Kong)
August 2008
Purpose: To evaluate the effect of autologous human serum (AHS) versus pooled human serum (PHS) versus foetal bovine serum (FBS) for growth of articular chondrocytes and formation of chondrocytefibrin constructs.
Methods: Experiments with monolayer culture expansion of human articular chondrocytes were performed using basic culture media supplemented with 10% AHS, PHS, or FBS. Growth kinetics and specific phenotypic expression of the serially expanded chondrocytes were evaluated.