Positive interactions between interferon and chemotherapy due to direct tumor action rather than effects on host drug-metabolizing enzymes.

The mechanism of increased antitumor activity when human lymphoblastoid interferon [HuIFN-alpha(Ly)] and the drugs cyclophosphamide and Adriamycin are used in combination on a human tumor xenograft in nude mice has been investigated. HuIFN-alpha(Ly) did not affect hepatic levels of the drug-metabolizing enzymes cytochrome P-450 or the glutathione S-transferases. In contrast, mouse interferon caused significant and differential changes in the isozymic forms of these enzymes.

Interferon production by cultured murine splenocytes in response to influenza virus-infected cells.

Murine splenic lymphocytes cultured for 18 h with either irradiated syngeneic cells infected with influenza A/PR/8/34 virus or irradiated allogeneic cells infected with the same virus release significant quantities of alpha interferon (IFN-alpha) in 24 h. Uninfected allogeneic cells, which are potent stimulators of both lymphocyte proliferation and cytolytic T lymphocytes, failed to induce detectable level of IFN under the same conditions. Cells derived from naive and virus-primed animals gave similar responses to these stimulae.

Herpes simplex virus persistence in mouse neuroblastoma (C 1300) cell cultures: role of interferon.

The Mp strain of herpes simplex virus type 1 (HSV1) induced a persistent infection in the mouse C 1300 neuronal cell line (clone N 115). C 1300 cultures infected at an MOI of 0.01 or 0.

Antiviral response of fibroblasts from familial Alzheimer's disease and Down's syndrome to human interferon-alpha.

The relative sensitivities of human fibroblast cultures from patients with familial Alzheimer's disease (AD), patients with Down's syndrome (Trisomy 21, T-21), and normal control (D-21) subjects, to the antiviral effect of human interferon-alpha (Hu-IFN-alpha) were compared. In confirmation of previous studies, T-21 cell lines showed significantly enhanced sensitivities to IFN compared with D-21 cells from normal controls. Fibroblast cultures from 2 familial AD patients belonging to one family exhibited sensitivities which fell within the range of response of normal D-21 cells.

Virus-host cell interaction in rat C6 glial cell cultures persistently infected with herpes simplex virus.

The virus-host cell interaction in a rat C6 glial cell line persistently infected with herpes simplex virus type 1 (HSV1) was examined. The C6 cultures infected at an MOI of 10 or 0.01 exhibited virally induced cytopathology and produced infectious virus and viral antigens for 108 and 50 days, respectively.