5-HT1B receptor modulation of the serotonin transporter in vivo: studies using KO mice.

The serotonin transporter (SERT) controls the strength and duration of serotonergic neurotransmission by the high-affinity uptake of serotonin (5-HT) from extracellular fluid. SERT is a key target for many psychotherapeutic and abused drugs, therefore understanding how SERT activity and expression are regulated is of fundamental importance. A growing literature suggests that SERT activity is under regulatory control of the 5-HT1B autoreceptor.

Multilayered films fabricated from plasmid DNA and a side-chain functionalized poly(beta-amino ester): surface-type erosion and sequential release of multiple plasmid constructs from surfaces.

Hydrolytically degradable polyamines can be used to fabricate multilayered polyelectrolyte films that erode and release DNA in aqueous environments. Past studies have investigated films fabricated from poly(beta-amino ester) 1 and the influence of polymer backbone structure on film erosion and the release of anionic polyelectrolytes. This investigation sought to characterize the influence of polymer side-chain structure on the stability of multilayered films in physiologically relevant media.

Ethanol inhibits clearance of brain serotonin by a serotonin transporter-independent mechanism.

Brain serotonin (5-HT) modulates the neural and behavioral effects of ethanol in a manner that remains poorly understood. Here we show that treatment with physiologically relevant (i.e.

Transport mechanisms governing serotonin clearance in vivo revealed by high-speed chronoamperometry.

High-speed chronoamperometry was used to determine the kinetics of clearance of exogenously applied serotonin (5-HT) in the dorsal raphe nucleus (DRN), dentate gyrus, CA3 region of the hippocampus or corpus callosum of anesthetized rats. Maximal velocity (Vmax) for 5-HT clearance was greatest in the DRN > dentate gyrus > CA3 > corpus callosum. Apparent affinity (K(T)) of the serotonin transporter (5-HTT) was similar in DRN and CA3 but greater in dentate gyrus and corpus callosum.

Serotonin (5-HT) transporter (SERT) function after graded destruction of serotonergic neurons.

The degree of occupancy of the serotonin transporter (SERT) by selective serotonin reuptake inhibitors (SSRIs) appears to be critical in determining therapeutic response. To gain insight into the extent of occupancy required to alter serotonergic neurotransmission we used high-speed chronoamperometry to determine the extent of serotonergic destruction required to reduce the clearance of exogenously administered serotonin from extracellular fluid in the CA3 region of the hippocampus. Rats were pretreated with various doses of 5,7-dihydroxytryptamine to produce either a low, intermediate or high loss of SERTs.