Genetics of human male infertility.

Infertility is defined as a failure to conceive in a couple trying to reproduce for a period of two years without conception. Approximately 15 percent of couples are infertile, and among these couples, male factor infertility accounts for approximately 50 percent of causes. Male infertility is a multifactorial syndrome encompassing a wide variety of disorders.

A386G transition in DAZL gene is not associated with spermatogenic failure in Tamil Nadu, South India.

The DAZ-like (DAZL) gene located on the short arm of autosomal chromosome 3 (3p24), an essential master gene for the premeiotic development of male and female germ cells, is the father of the Y-chromosome DAZ gene cluster and encodes for RNA-binding proteins. Reported instances of positive association of DAZL gene mutations with infertility in men have been found in a Taiwanese population but not in Caucasians. There is no study from Tamil Nadu, South India, to demonstrate the role of DAZL gene in male infertility; we, therefore, analyzed a total of 287 men, including 147 infertile and 140 normozoospermic fertile controls from rural areas of Tamil Nadu, South India, to assess the phenotypic effect of DAZL mutations in this region of the world.

Male infertility: polymerase chain reaction-based deletion mapping of genes on the human chromosome.

Introduction: Y chromosome microdeletions are common in about 10-15 percent of men with azoospermia or severe oligospermia. These microdeletions are too small to be detected by karyotyping. They can be easily identified using polymerase chain reaction (PCR).

Hepatotoxicity of eugenol.

EUGENOL a widely used pharmaceutical agent proceeds toxicity on inhalation in rats. Two different doses 20 & 30 μg/100g body weight/ day of eugenol were given intramuscularly to male albino rats for 10 days and the liver function was assessed by measuring the specific enzyme activities, and total and differential bilirubin concentration. There was an appreciable increase in total as well as differential bilirubin fractions and a dose dependent increase was noticed in the activities of alkaline phosphatase, transminases, lactate dehyrogenase with a decrease in the activity of glytamyl transferase activity, and suggesting eugenol to have a toxic effect on liver.

Effects of anethole on seminal vesicle of albino rats.

Administration of anethole at 10 and 50 mg doses caused significant reduction in seminal vesicle weight, RNA and protein concentrations and contents and acid phosphates activity. However, there was significant increase in DNA concentrations and in the activities of alkaline phosphates and lactate dehydrogenase. The effects were more pronounced in the group receiving 50 mg of anethole.