Publications by authors named "S M Szczepanek"
Article Synopsis
- * Current vaccines help a bit but aren't very effective or safe, so scientists created a new type of vaccine using special parts of the germ.
- * The new vaccine showed good results in chickens, especially when combined with a specific ingredient that helped reduce illness and damage in their throats.
This study investigates the roles of RUVBL1 and HIF1A in ccRCC development and explores their clinical significance as prognostic biomarkers. mRNA and protein expressions were analyzed using TCGA data and an institutional tissue cohort, respectively. Correlations with clinicopathological parameters and patient outcomes were assessed.
View Article and Find Full Text PDFThe necessity for multiple injections and cold-chain storage has contributed to suboptimal vaccine utilization, especially in pandemic situations. Thermally-stable and single-administration vaccines hold a great potential to revolutionize the global immunization process. Here, a new approach to thermally stabilize protein-based antigens is presented and a new high-throughput antigen-loading process is devised to create a single-administration, pulsatile-release microneedle (MN) patch which can deliver a recombinant SARS-CoV-2 S1-RBD protein-a model for the COVID-19 vaccine.
View Article and Find Full Text PDFDevelopment of an effective vaccine for Mycoplasma pneumoniae has been hindered by reports of Vaccine Enhanced Disease (VED) in test subjects vaccinated and challenged in studies conducted in the 1960s. The exact mechanism of disease exacerbation has yet to be fully described, but host immune responses to Lipid-Associated Membrane Proteins (LAMPs) lipoprotein lipid moieties have been implicated. LAMPs-induced exacerbation appears to involve helper T cell recall responses, due in part to their influence on neutrophil recruitment and subsequent inflammatory responses in the lung.
View Article and Find Full Text PDFArticle Synopsis
- Bacterial lipoproteins can cause strong immune reactions but are often overlooked in vaccine development.
- Vaccinating mice with Mycoplasma pneumoniae lipoproteins led to an immune response that unexpectedly worsened the disease after exposure to the virus.
- Increased levels of inflammatory cytokines and neutrophils were observed in the lungs, indicating that IL-17A plays a crucial role in this maladaptive immune response, while neutrophils contributed negatively to the disease's severity.
- The research highlights the need to consider potential negative immune responses when designing vaccines targeting bacterial infections.