Molecular analysis of neutrophil spontaneous apoptosis reveals a strong role for the pro-apoptotic BH3-only protein Noxa.

Neutrophils enter the peripheral blood from the bone marrow and die after a short time. Molecular analysis of spontaneous neutrophil apoptosis is difficult as these cells die rapidly and cannot be easily manipulated. We use conditional Hoxb8 expression to generate mouse neutrophils and test the regulation of apoptosis by extensive manipulation of B-cell lymphoma protein 2 (Bcl-2)-family proteins.

FADD and the NF-kappaB family member Bcl-3 regulate complementary pathways to control T-cell survival and proliferation.

Fas-associated protein with death domain/mediator of receptor induced toxicity (FADD/MORT1) was first described as a transducer of death receptor signalling but was later recognized also to be important for proliferation of T cells. B-cell lymphoma 3 (Bcl-3) is a relatively little understood member of the nuclear factor (NF)-kappaB family of transcription factors. We recently found that Bcl-3 is up-regulated in T cells from mice where FADD function is blocked by a dominant negative transgene (FADD-DN).

Five year study of rotavirus gastroenteritis in Bulgaria.

We tested by electron microscopy 7,530 samples from children admitted to different hospitals in Sofia between February 1981 and February 1986; from these, rotaviruses were found in 725 (9.6%) faecal samples. Electron microscopic analysis of 264 samples from 181 children admitted to the hospital of infectious diseases in Shumen between December 1984 to February 1986 revealed rotaviruses in 120 (66.