Paracetamol (acetaminophen) analysis by high performance liquid chromatography: interference studies and comparison with an enzymatic procedure.

A simple, robust, high performance liquid chromatographic method for serum paracetamol determination in both the therapeutic and overdose concentration ranges is described. The within- and between-batch precision of the method was better than CV 5% at both micromolar and millimolar concentrations, and recovery of added paracetamol from drug-free serum exceeded 96%. No interference from any of the other drugs, drug metabolites, and dietary constituents tested was found.