A Comparative Study on the Effects of the Lysine Reagent Pyridoxal 5-Phosphate and Some Thiol Reagents in Opening the Tl-Induced Mitochondrial Permeability Transition Pore.

Lysine residues are essential in regulating enzymatic activity and the spatial structure maintenance of mitochondrial proteins and functional complexes. The most important parts of the mitochondrial permeability transition pore are F1F0 ATPase, the adenine nucleotide translocase (ANT), and the inorganic phosphate cotransporter. The ANT conformation play a significant role in the Tl-induced MPTP opening in the inner membrane of calcium-loaded rat liver mitochondria.

The Joint Influence of Tl and Thiol-Modifying Agents on Rat Liver Mitochondrial Parameters In Vitro.

Recent data have shown that the mitochondrial permeability transition pore (MPTP) is the complex of the Ca-modified adenine nucleotide translocase (ANT) and the Ca-modified ATP synthase. We found in a previous study that ANT conformational changes may be involved in Tl-induced MPTP opening in the inner membrane of Ca-loaded rat liver mitochondria. In this study, the effects of thiol-modifying agents (eosin-5-maleimide (EMA), fluorescein isothiocyanate (FITC), Cu(o-phenanthroline) (Cu(OP)), and embelin (Emb)), and MPTP inhibitors (ADP, cyclosporine A (CsA), n-ethylmaleimide (NEM), and trifluoperazine (TFP)) on MPTP opening were tested simultaneously with increases in swelling, membrane potential (ΔΨ) decline, decreases in state 3, 4, and 3U (2,4-dinitrophenol-uncoupled) respiration, and changes in the inner membrane free thiol group content.

Mitochondrial mechanisms by which gasotransmitters (HS, NO and CO) protect cardiovascular system against hypoxia.

Over past few years, there has been a dramatic increase in studying physiological mechanisms of the activity of various signaling low-molecular molecules that directly or indirectly initiate adaptive changes in the cardiovascular system cells (CVSC) to hypoxia. These molecules include biologically active endogenous gases or gasotransmitters (HS, NO and CO) that influence on many cellular processes, including mitochondrial biogenesis, oxidative phosphorylation, K/Ca exchange, contractility of cardiomyocytes (CM) and vascular smooth muscle cells (VSMC) under conditions of oxygen deficiency. The present review focuses on the mechanistic role of the gasotransmitters (NO, HS, CO) in cardioprotection.

Compact generators of high-power fast-rising pulses with closing switches in the form of reversely switched dynistors.

This paper presents the results of a study of reversely switched dynistors (RSDs) with an operating voltage of 2.3 kV and a structure diameter of 12 mm in an unconventional mode, when they commutate current pulses with very short durations (a few microseconds). In this mode, we studied the dependence of the switching energy loss on the amount of charge that passes through the RSDs during the flow of the control current, which is reversed with respect to the main current.