[A synthetic medium containing growth factors for the cultivation of producer cell lines].

A synthetic medium with low serum contents has been composed. As basic substitutes for serum a mixture containing a fibroblast growth factor, insulin, albumin and fibronectin is proposed. It allows to carry out a long-term cultivation of RH-PA cells, which are producers of a plasminogen activator, both in static conditions and on microcarriers.

[Heparin-bound growth factors produced by the established RH-PA cell line].

Two types of heparin-bound growth factors (HBGF) are isolated from serum-free culture media of RH-PA cells, which are effective producers of urokinase type plasminogen activator, and from a lysate of these cells. According to affinity to heparin, molecular weight and cells proliferation stimulation, these HBGF are similar to basic and acidic fibroblast growth factors. It is shown that the obtained preparations, produced by RH-PA cells at 2 pg/ml concentration and more, exert mitogenic effect towards RH-PA and NIH 3T3 cells.